黃尉倫

Wei-Lun Hwang

聯絡方式

☏ +886-2-28267000 ext: 65833 (Lab) / 65832 (Office)

✉ wlhwang@nycu.edu.tw

〒 Department of Biotechnology and Laboratory Science in Medicine, National Yang Ming Chiao Tung University, No. 155, Sec. 2, Linong St., Beitou District, Taipei 112, Taiwan

學歷

Ph.D, Institute of Biochemistry and Molecular Biology (Taiwan International Graduate Program, TIGP),          National Yang Ming University, Taipei, Taiwan

M.S., Institute of Microbiology and Immunology, National Yang Ming University, Taipei, Taiwan 

B.S., School of Medical Laboratory Science and Technology, Taipei Medical University, Taipei, Taiwan

經歷

  • Feb 2022-present, Associate Professor, Department of Biotechnology and Laboratory Science in Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan
  • Aug 2018-Jan 2022, Assistant Professor, Department of Biotechnology and Laboratory Science in Medicine, National Yang Ming University, Taipei, Taiwan
  • Oct 2015-Jul 2018, Assistant Professor, The Ph.D Program for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, Taipei, Taiwan
  • Oct 2012-Jul 2015, Post-Doctoral Fellow, Institute of Microbiology and Immunology and Institute of Clinical Medicine, National Yang Ming University, Taipei, Taiwan
  • 醫事檢驗師國家檢覈考試典試委員/命題委員 (臨床生物化學/ 臨床鏡檢學)
  • 計畫審查委員(國科會科技及專題計畫審查委員、臺大醫院、臺北榮民總醫院計畫審查委員等)
  • 期刊論文審查委員(Oncogene, Journal of Biomedical Science, International Journal of Nanomedicine, Aging, Angiogenesis, Biomedicine & Pharmacotherapy, Cancer Biomarkers, Clinical and Translational Medicine, Mathematical Biosciences and Engineering); Member in the Asian Council of Science Editors (2021).

研究方向

腫瘤細胞的去分化程度及幹細胞特性與病患不良預後息息相關。我們從大腸直腸癌檢體和細胞株中利用無血清培養平台分離出腸癌幹細胞。透過微陣列分析和次世代定序腸癌幹細胞的轉錄體解碼，我們發現腸癌幹細胞為 CD44(+)/CD166(+)之癌幹細胞亞群，並具有上皮間質轉化的特性。轉錄因子Snail 是腸癌幹細胞的重要調節因子。Snail-IL8 信號軸和 Snail-miR-146a-Numb 路徑分別維持了Nanog/OCT4等胚胎幹細胞基因的表達與腸癌幹細胞的對稱性細胞分裂。在大腸直腸癌患者中，Snail-IL8與腸癌幹細胞標誌CD44共表達，Snail(+)Numb(-/neg)病患亞群亦與標靶藥物Erbitux抗性相關。腸癌幹細胞可藉由RAB27B活化來分泌更多的胞外體，透過胞外體非編碼RNA來教育嗜中性球以形成免疫抑制性腫瘤微環境並導致腫瘤惡化。我們的研究證實腫瘤微環境是影響腫瘤治療成效的重要關鍵，更是開發潛在腫瘤標靶藥物及生物標記的重要方向。

Cancer cells possess characters similar to healthy stem cells, and the degree of cell dedifferentiation is correlated with poor prognosis. We isolated cancer stem cells (CSCs) as sphere-derived cancer stem cells (SDCSCs) from primary colorectal cancer (CRC) tissues and cell lines for characterization in vitro and in vivo. Microarray analysis and next-generation sequencing of the SDCSCs were used to elucidate their transcriptome and miRNome for exploring their enriched biological activities. We found expanded SDCSCs were enriched with CD44(+)/CD166(+) subpopulations and had undergone epithelial-mesenchymal transition (EMT) with Snail being the predominant regulator. The Snail-IL8 signal axis and Snail-miR-146a-Numb circuits were demonstrated to maintain Nanog/OCT4 expression for maintaining tumor niches and Wnt signal cassettes for symmetric stem cell division, respectively. In colorectal cancer patients, Snail and IL8 were coexpressed with the stem cell marker CD44 and the SnailHighNumbLow profile was correlated with Erbitux resistance. Exosomal non-coding RNAs of SDCSCs further orchestrated a pro-tumoral, immunosuppressive tumor microenvironment (TME) by activating host neutrophils. Dr. Hwang’s studies reveal the presence of a complex interactome of CSCs, the potential therapeutic target for cancer treatment.

Research Interests:

腫瘤微環境 (Tumor Microenvironment); 腫瘤宿主交互作用(Tumor-Host Interactions); 癌幹細胞 (Cancer Stem Cells); 腫瘤轉移(Cancer Metastasis)

胞外體 (Extracellular Vesicles); 生物標記開發 (Biomarker Discovery); 非編碼核糖核酸 (Noncoding RNAs); 治療抗性 (Therapeutic Resistance)

透過體學研究策略了解腫瘤微環境中的癌細胞與免疫細胞如何溝通
癌幹細胞外泌體(Exosomes)教育嗜中性白血造成腸癌惡化
外泌體(Exosomes)在腫瘤微環境中的角色及治療策略

奈米粒徑(外泌體)檢測服務

透過奈米粒子分析儀(Exoid nanoparticle analyzer)，將粒子逐一通過奈米孔洞，並使用國際胞外體學會(ISEV)認可之可調式電阻脈衝感應(Tunable resistive pulse sensing/ TRPS)技術來分析奈米粒子的大小及濃度，也可偵測每顆粒子的界達電位。進行生物性外泌體或奈米材料等檢測前請參閱檢測範疇(如下: 附件一)，再填寫奈米粒徑分析儀檢測申請單(如下: 附件二)及繳費(如下:附件三)或轉帳以完成送件。示範結果報告如下附件四。歡迎使用者與我們洽談檢測服務與相關的產學合作計畫。
附件一：檢測範疇
附件二：服務申請單
附件三：檢測繳費單
附件四：示範報告

榮譽與獎項

2023 吳大猷先生紀念獎計畫(多年期)
2023 生醫工程學院 EMI教師教學獎
2021 科技部吳大猷先生紀念獎(腫瘤領域)
2021 沈力揚教授醫學教育獎學紀念基金會研究與進修獎助
2021 科技部優秀年輕學者研究計畫(多年期)
2019 紀念沈力揚教授講師級研究與進修獎助
2019 參與陽明大學圖書館Nature封面影展影片拍攝
2017-2020 科技部優秀年輕學者研究計畫(多年期)
2014 科技部103年度博士後研究人員學術著作獎
2013 第8屆永信李天德醫藥科技獎/傑出論文獎
2013 第18屆台灣癌症聯合學術年會(TJCC)基礎轉譯組,優等獎
2012 第17屆 台灣癌症聯合學術年會(TJCC)基礎轉譯組,特等獎
2012 林榮耀教授學術教育基金會優秀論文獎
2012 Merck Millipore Asia Bioforum (Taipei)銀牌獎
2011-12 國立陽明大學學術論文比賽優等獎/陽明博士班優秀論文獎學金
2019 A*STAR, Singapore, Cell Symposia: Single Cells: Technology to Biology, Singapore, 2019 (科技部計畫補助)
2014 Institute Curie, France, Non-Coding Genome (教師及研究人員赴國外研究訪問, 陽明大學補助) 
2012 Sitges, Spain, Cell Symposia, “Functional RNAs”  (國科會補助)
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2024 指導研究生趙慧青獲112學年度學術論文研討會海報展示佳作
2024 指導研究生趙慧青獲醫技系論文比賽第二名
2024 研究生林宥彤獲國科會學生參與國際會議補助
2023 指導研究生曾馨萱獲111學年度學術論文研討會海報展示佳作
2023 指導研究生曾馨萱獲醫技系論文比賽第一名&最佳人氣獎
2022 指導陳慧君同學獲科技部大專生專題研究計畫
2021 指導新創團隊獲教育部生醫產業與新農業創新創業競賽 佳作獎
2021 指導研究生林巧雯獲醫技系論文比賽第一名
2020 指導研究生王薏荃獲醫技系論文比賽第三名
2019 指導生技檢驗部優勝團隊ProLung生物科技公司-新型非侵入性肺癌篩檢平台
2016 指導藥學系林煒樺獲北醫大105年度大專生專題計畫獎第二名

研究著作

1. CT45A1-mediated MLC2 (MYL9) phosphorylation promotes natural killer cell resistance and outer cell fate in a cell-in-cell structure, potentiating the progression of microsatellite instability-high colorectal cancer. Hao-Wei Teng , Hsiang-Yueh Huang , Chun-Chi Lin  , Yuh-Ching Twu , Wen-Hao Yang  , Wen-Chun Lin, Hsin-Yi Lan , Yen-Yu Lin , Wei-Lun Hwang (Mol Oncol. 2024 Sep 25. doi: 10.1002/1878-0261.13736.)

2. Ganoderma microsporum immunomodulatory protein combined with KRASG12C inhibitor impedes intracellular AKT/ERK network to suppress lung cancer cells with KRAS mutation. Wei-Jyun Hua, Wei-Lun Hwang, Hsin Yeh, Zhi-Hu Lin, Wei-Hung Hsu, Tung-Yi Lin. Int J Biol Macromol. 2024 Feb;259(Pt 2):129291. (IF: 8.2 ; 5/86 (Q1), Polymer Science)

3. Ganoderma microsporum immunomodulatory protein as an extracellular epidermal growth factor receptor (EGFR) degrader for suppressing EGFR-positive lung cancer cells. Wei-Jyun Hua, Hsin Yeh, Zhi-Hu Lin, Ai-Jung Tseng, Li-Chen Huang, Wei-Lun Qiu, Tsung-Hsi Tu, Ding-Han Wang, Wei-Hung Hsu, Wei-Lun Hwang, Tung-Yi Lin. Cancer Lett. 2023 Oct 19:216458. (IF: 9.7; 31/318 (Q1), Oncology)

4. Haematopoietic cell-derived exosomes in cancer development and therapeutics: From basic science to clinical practice. Wen-Chun Lin†, You-Tong Lin†, Hui-Ching Chao, Yen-Yu Lin, Wei-Lun Hwang*. Clin Transl Med. 2023 Oct;13(10):e1448. (IF: 10.6; 17/189 (Q1), Medicine, Research & Experimental)

5. T-cell exhaustion and stemness in antitumor immunity: characteristics, mechanisms, and implications. Xiaoxia Chi†, Shahang Luo†, Peng Ye†, Wei-Lun Hwang, Jong-ho Cha* , Xiuwen Yan* and Wen-Hao Yang*. Front Immunol. 2023 Feb 20;14:1104771. (IF: 8.786; 43/178 (Q1), Immunology)

6. Molecular actions of exosomes and their theragnostics in colorectal cancer: current findings and limitations. Wen‑Chun Lin, Chun‑Chi Lin, Yen‑Yu Lin, Wen‑Hao Yang, Yuh‑Ching Twu, Hao‑Wei Teng,* Wei‑Lun Hwang.* Cellular Oncology, 2022 Dec;45(6):1043-1052. (IF: 7.051; 54/318 (Q1), Oncology)

7. Insulin-Like Growth Factor 2 mRNA-Binding Protein 1 (IGF2BP1) Is a Prognostic Biomarker and Associated with Chemotherapy Responsiveness in Colorectal Cancer. Hung-Ming Chen, Chun-Chi Lin, Wei-Shone Chen, Jeng-Kai Jiang, Shung-Haur Yang, Shih-Ching Chang, Ching-Liang Ho, Chung-Chi Yang, Shih-Ching Huang, Yee Chao, Tsai-Tsen Liao, Wei-Lun Hwang* and Hao-Wei Teng*, Int. J. Mol. Sci. 2021, 22(13), 6940;  (IF: 5.92; 67/298 (Q1), Biochemistry & Molecular Biology)

8. Curcumin Suppresses TGF-β1-Induced Myofibroblast Differentiation and Attenuates Angiogenic Activity of Orbital Fibroblasts. Wei-Kuang Yu, Wei-Lun Hwang, Yi-Chuan Wang, Chieh-Chih Tsai and Yau-Huei Wei. Int. J. Mol. Sci. 2021, 22(13), 6829;  (IF: 5.92; 67/298 (Q1), Biochemistry & Molecular Biology)

9. Human ribonuclease 1 serves as a secretory ligand of ephrin A4 receptor and induces breast tumor initiation. Lee HH†, Wang YN†, Yang WH†, Xia W, Wei YK , Chan LC, Wang YH, Jiang Z, Xu SP , Yao J , Qiu YF, Hsu YH, Hwang WL, Yan MS , Cha JH , Hsu Jennifer , Shen J , Ye Y, Wu X , Hou MF , Tseng LM , Wang SC , Pan MR , Yang CH , Wang YL , Yamaguchi H, Pang D , Hortobagyi G, Yu Dihua, Hung MC. Nat Commun. 2021, Nat Commun. 2021 May 13;12(1):2788 (IF: 14.91; 4/73 (Q1), Multidisciplinary Sciences)

10. The microRNA-210-stathmin1 axis decreases cell stiffness to facilitate the invasiveness of colorectal cancer stem cells. Liao TT, Cheng WC, Yang CY, Chen YQ, Su SH, Yeh TY, Lan HY, Lee CC, Lin HH, Lin CC, Lu RH, Chiou ET, Jiang JK, Hwang WL*. Cancers 2021, Apr 12;13(8):1833. (IF: 6.63; 51/242 (Q1), Oncology)

11. Roles of microRNAs in regulating cancer stemness in head and neck cancers. Fitriana Melysa †, Hwang WL†, Chan PY, Hsueh TY and Liao TT. Cancers 2021, 13(7), 1742;  (IF: 6.63; 51/242 (Q1), Oncology)

12. Muscle atrophy-related myotube-derived exosomal microRNA in neuronal dysfunction: Targeting both coding and long noncoding RNAs. Yang CP, Yang WS, Wong YH, Wang KH, Teng YC, Chang MH, Liao KH, Nian FS, Chao CC, Tsai JW, Hwang WL, Lin MW, Tzeng TY, Wang PN, Campbell M, Chen LK, Tsai TF, Chang PC, Kung HJ. Aging Cell. 2020 May;19 (5):e13107. (IF: 9.30; 4/53 (Q1), Geriatrics and Gerontology)

13. RAB27B-activated secretion of stem-like tumor exosomes delivers the biomarker microRNA-146a-5p, which promotes tumorigenesis and associates with an immunosuppressive tumor microenvironment in colorectal cancer. Cheng WC, Liao TT, Lin CC, Yuan LE, Lan HY, Lin HH, Teng HW, Chang HC, Lin CH, Yang CY, Huang SC, Jiang JK, Yang SH, Yang MH, Hwang WL*. Int J Cancer. 2019 Oct 15;145 (8):2209-2224. (IF: 7.39; 42/242 (Q1), Oncology)

14. Targeting non-muscle myosin II promotes corneal endothelial migration through regulating lamellipodial dynamics. Ho WT, Chang JS, Chou SF, Hwang WL, Shih PJ, Chang SW, Yang MH, Jou TS, Wang IJ. J Mol Med (Berl). 2019 Sep;97(9):1345-1357. (IF: 4.59; 48/175 (Q2), Genetics and Heredity)

15. Tumor stem-like cell-derived exosomal RNAs prime neutrophils for facilitating tumorigenesis of colon cancer. Hwang WL*, Lan HY, Cheng WC, Huang SC, Yang MH. J Hematol Oncol. 2019 Jan 25;12 (1):10. (IF: 17.38; 3/76 (Q1), Hematology)

16. Macrophage-secreted interleukin-35 regulates cancer cell plasticity to facilitate metastatic colonization. Lee CC, Lin JC, Hwang WL, Kuo YJ, Chen HK, Tai SK, Lin CC, Yang MH. Nat Commun. 2018 Sep 14;9(1):3763. (IF: 14.91; 4/73 (Q1), Multidisciplinary Sciences)

17. Endothelial angiogenesis is directed by RUNX1T1-regulated VEGFA, BMP4 and TGF-β2 expression. Liao KH, Chang SJ, Chang HC, Chien CL, Huang TS, Feng TC, Lin WW, Shih CC, Yang MH, Yang SH, Lin CH, Hwang WL*, Lee OK*. PLoS One. 2017 Jun 22;12(6):e0179758. (IF=2.74; 27/ 71, Multidisciplinary sciences)

18. Lymphotoxin-β Interacts with Methylated EGFR to Mediate Acquired Resistance to Cetuximab in Head and Neck Cancer. Hsu DS#, Hwang WL#, Yuh CH, Chu CH, Ho YH, Chen PB, Lin HS, Lin HK, Wu SP, Lin CY, Hsu WH, Lan HY, Wang HJ, Tai SK, Hung MC, Yang MH. Clin Cancer Res. 2017 Aug 1;23(15):4388-4401. (IF=10.10; 17/244; Oncology)

19. Numb is involved in the non-random segregation of subcellular vesicles in colorectal cancer stem cells. Hwang WL, Yang MH. Cell Cycle. 2016 Oct17;15 (20):2697-703. (IF=3.39; 95/195; Cell Biology)

20. MicroRNA-146a directs the symmetric division of Snail-dominant colorectal cancer stem cells. Hwang WL, Jiang JK, Yang SH, Huang TS, Lan HY, Teng HW, Yang CY, Tsai YP, Lin CH, Wang HW, Yang MH. Nat Cell Biol. 2014 Mar;16(3):268-80. (IF=20.04; 8/195; Cell Biology)

21. SNAIL regulates interleukin-8 expression, stem cell-like activity, and tumorigenicity of human colorectal carcinoma cells. Hwang WL, Yang MH, Tsai ML, Lan HY, Su SH, Chang SC, Teng HW, Yang SH, Lan YT, Chiou SH, Wang HW. Gastroenterology. 2011 Jul;141(1):279-91, 291.e1-5. (IF=17.37; 4/88, Gastroenterology and Hepatology)

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