{"id":291,"date":"2021-03-25T01:21:16","date_gmt":"2021-03-25T01:21:16","guid":{"rendered":"http:\/\/dmt.nycu.edu.tw\/?page_id=291"},"modified":"2025-11-13T11:40:19","modified_gmt":"2025-11-13T03:40:19","slug":"%e9%be%94%e6%80%9d%e8%b1%aa","status":"publish","type":"page","link":"https:\/\/dmt.nycu.edu.tw\/en\/%e9%be%94%e6%80%9d%e8%b1%aa\/","title":{"rendered":"\u9f94\u601d\u8c6a"},"content":{"rendered":"<div data-elementor-type=\"wp-page\" data-elementor-id=\"291\" class=\"elementor elementor-291\">\n\t\t\t\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-2fb70766 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"2fb70766\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-76c7c2b9\" data-id=\"76c7c2b9\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-6fe03b26 elementor-widget elementor-widget-heading\" data-id=\"6fe03b26\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.11.1 - 15-02-2023 *\/\n.elementor-heading-title{padding:0;margin:0;line-height:1}.elementor-widget-heading .elementor-heading-title[class*=elementor-size-]>a{color:inherit;font-size:inherit;line-height:inherit}.elementor-widget-heading .elementor-heading-title.elementor-size-small{font-size:15px}.elementor-widget-heading .elementor-heading-title.elementor-size-medium{font-size:19px}.elementor-widget-heading .elementor-heading-title.elementor-size-large{font-size:29px}.elementor-widget-heading .elementor-heading-title.elementor-size-xl{font-size:39px}.elementor-widget-heading .elementor-heading-title.elementor-size-xxl{font-size:59px}<\/style><h2 class=\"elementor-heading-title elementor-size-default\">\u9f94\u601d\u8c6a<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-4b76d19e elementor-widget elementor-widget-text-editor\" data-id=\"4b76d19e\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.11.1 - 15-02-2023 *\/\n.elementor-widget-text-editor.elementor-drop-cap-view-stacked .elementor-drop-cap{background-color:#818a91;color:#fff}.elementor-widget-text-editor.elementor-drop-cap-view-framed .elementor-drop-cap{color:#818a91;border:3px solid;background-color:transparent}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap{margin-top:8px}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap-letter{width:1em;height:1em}.elementor-widget-text-editor .elementor-drop-cap{float:left;text-align:center;line-height:1;font-size:50px}.elementor-widget-text-editor .elementor-drop-cap-letter{display:inline-block}<\/style>\t\t\t\t<p>Szu-Hao\u00a0Kung<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7939566b elementor-widget elementor-widget-image\" data-id=\"7939566b\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.11.1 - 15-02-2023 *\/\n.elementor-widget-image{text-align:center}.elementor-widget-image a{display:inline-block}.elementor-widget-image a img[src$=\".svg\"]{width:48px}.elementor-widget-image img{vertical-align:middle;display:inline-block}<\/style>\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"238\" height=\"286\" src=\"https:\/\/dmt.nycu.edu.tw\/wp-content\/uploads\/2021\/03\/\u9f94\u8001\u5e2b.jpg\" class=\"attachment-large size-large wp-image-313\" alt=\"\" loading=\"lazy\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-66 elementor-top-column elementor-element elementor-element-78bf17d\" data-id=\"78bf17d\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-56a53b81 elementor-widget elementor-widget-heading\" data-id=\"56a53b81\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">\u806f\u7d61\u65b9\u5f0f<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1016e6e3 elementor-widget elementor-widget-text-editor\" data-id=\"1016e6e3\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>\u260f\u00a002-28267034<\/p>\n<p>\u2709\u00a0<a href=\"mailto:szkung@ym.edu.tw\">szkung@nycu.edu.tw<\/a><\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-3cdceca4 elementor-widget elementor-widget-heading\" data-id=\"3cdceca4\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">\u5b78\u6b77<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2dbb2565 elementor-widget elementor-widget-text-editor\" data-id=\"2dbb2565\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>\u5357\u4f5b\u7f85\u91cc\u9054\u5927\u5b78<br \/>\u5fae\u751f\u7269\u8207\u514d\u75ab\u5b78 \u535a\u58eb<\/p><p>\u570b\u7acb\u967d\u660e\u5927\u5b78<br \/>\u91ab\u4e8b\u6280\u8853 \u5b78\u58eb.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-5bf680d7 elementor-widget elementor-widget-heading\" data-id=\"5bf680d7\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">\u73fe\u8077\u8207\u7d93\u6b77<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-379a4143 elementor-widget elementor-widget-text-editor\" data-id=\"379a4143\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>\u570b\u7acb\u967d\u660e\u5927\u5b78<br \/>\u91ab\u5b78\u751f\u7269\u6280\u8853\u66a8\u6aa2\u9a57\u5b78\u7cfb \u526f\u6559\u6388<\/p><p>\u570b\u7acb\u967d\u660e\u5927\u5b78<br \/>\u91ab\u5b78\u751f\u7269\u6280\u8853\u66a8\u6aa2\u9a57\u5b78\u7cfb \u52a9\u7406\u6559\u6388<\/p><p>\u7f8e\u570b\u8cd3\u5dde\u5927\u5b78<br \/>\u75c5\u7406\u7cfb \u535a\u58eb\u5f8c\u7814\u7a76<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-72aaf70f elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"72aaf70f\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-5fa02061\" data-id=\"5fa02061\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-79012af9 elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"79012af9\" data-element_type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.11.1 - 15-02-2023 *\/\n.elementor-widget-divider{--divider-border-style:none;--divider-border-width:1px;--divider-color:#2c2c2c;--divider-icon-size:20px;--divider-element-spacing:10px;--divider-pattern-height:24px;--divider-pattern-size:20px;--divider-pattern-url:none;--divider-pattern-repeat:repeat-x}.elementor-widget-divider .elementor-divider{display:flex}.elementor-widget-divider .elementor-divider__text{font-size:15px;line-height:1;max-width:95%}.elementor-widget-divider .elementor-divider__element{margin:0 var(--divider-element-spacing);flex-shrink:0}.elementor-widget-divider .elementor-icon{font-size:var(--divider-icon-size)}.elementor-widget-divider .elementor-divider-separator{display:flex;margin:0;direction:ltr}.elementor-widget-divider--view-line_icon .elementor-divider-separator,.elementor-widget-divider--view-line_text .elementor-divider-separator{align-items:center}.elementor-widget-divider--view-line_icon .elementor-divider-separator:after,.elementor-widget-divider--view-line_icon .elementor-divider-separator:before,.elementor-widget-divider--view-line_text .elementor-divider-separator:after,.elementor-widget-divider--view-line_text .elementor-divider-separator:before{display:block;content:\"\";border-bottom:0;flex-grow:1;border-top:var(--divider-border-width) var(--divider-border-style) var(--divider-color)}.elementor-widget-divider--element-align-left .elementor-divider .elementor-divider-separator>.elementor-divider__svg:first-of-type{flex-grow:0;flex-shrink:100}.elementor-widget-divider--element-align-left .elementor-divider-separator:before{content:none}.elementor-widget-divider--element-align-left .elementor-divider__element{margin-left:0}.elementor-widget-divider--element-align-right .elementor-divider .elementor-divider-separator>.elementor-divider__svg:last-of-type{flex-grow:0;flex-shrink:100}.elementor-widget-divider--element-align-right .elementor-divider-separator:after{content:none}.elementor-widget-divider--element-align-right .elementor-divider__element{margin-right:0}.elementor-widget-divider:not(.elementor-widget-divider--view-line_text):not(.elementor-widget-divider--view-line_icon) .elementor-divider-separator{border-top:var(--divider-border-width) var(--divider-border-style) var(--divider-color)}.elementor-widget-divider--separator-type-pattern{--divider-border-style:none}.elementor-widget-divider--separator-type-pattern.elementor-widget-divider--view-line .elementor-divider-separator,.elementor-widget-divider--separator-type-pattern:not(.elementor-widget-divider--view-line) .elementor-divider-separator:after,.elementor-widget-divider--separator-type-pattern:not(.elementor-widget-divider--view-line) .elementor-divider-separator:before,.elementor-widget-divider--separator-type-pattern:not([class*=elementor-widget-divider--view]) .elementor-divider-separator{width:100%;min-height:var(--divider-pattern-height);-webkit-mask-size:var(--divider-pattern-size) 100%;mask-size:var(--divider-pattern-size) 100%;-webkit-mask-repeat:var(--divider-pattern-repeat);mask-repeat:var(--divider-pattern-repeat);background-color:var(--divider-color);-webkit-mask-image:var(--divider-pattern-url);mask-image:var(--divider-pattern-url)}.elementor-widget-divider--no-spacing{--divider-pattern-size:auto}.elementor-widget-divider--bg-round{--divider-pattern-repeat:round}.rtl .elementor-widget-divider .elementor-divider__text{direction:rtl}.e-con-inner>.elementor-widget-divider,.e-con>.elementor-widget-divider{width:var(--container-widget-width,100%);--flex-grow:var(--container-widget-flex-grow)}<\/style>\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2634b50f elementor-widget elementor-widget-heading\" data-id=\"2634b50f\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">\u7814\u7a76\u9818\u57df <\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-4488abb9 elementor-widget elementor-widget-text-editor\" data-id=\"4488abb9\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<h4>\u8178\u75c5\u6bd2\u53ca\u51a0\u72c0\u75c5\u6bd2\u7684\u81f4\u75c5\u6a5f\u5236\u53ca\u85e5\u7269\u958b\u767c\u3002<\/h4><p>\u00a0 \u00a0 \u00a0\u4eba\u985e\u8178\u75c5\u6bd2\u662f\u5c0f\u5152\u79d1\u7684\u91cd\u8981\u81f4\u75c5\u539f\u3002\u53f0\u7063\u5730\u5340\u5728\u904e\u53bb20\u9918\u5e74\u7206\u767c\u4e86\u6578\u6ce2\u5927\u5c0f\u4e0d\u4e00\u7684\u8178\u75c5\u6bd2\u6d41\u884c\uff0c\u800c\u8178\u75c5\u6bd271\u578b\u5247\u662f\u9020\u6210\u91cd\u75c7\u7684\u4e3b\u8981\u56e0\u5b50\u3002\u7136\u800c\uff0c\u81e8\u5e8a\u4e0a\u76ee\u524d\u9084\u6c92\u6709\u6297\u75c5\u6bd2\u85e5\u6cbb\u7642\u8178\u75c5\u6bd271\u578b\uff0c\u800c\u75ab\u82d7\u7684\u767c\u5c55\u50c5\u4fb7\u9650\u65bc\u8178\u75c5\u6bd271\u578b\uff0c\u5176\u4ed6\u578b\u5225\u7684\u8178\u75c5\u6bd2\u5247\u5c1a\u672a\u6709\u75ab\u82d7\u3002\u56e0\u6b64\uff0c\u6211\u5011\u5be6\u9a57\u5ba4\u9577\u671f\u4ee5\u4f86\u7814\u7a76\u8178\u75c5\u6bd2\u7684\u81f4\u75c5\u6a5f\u5236\u53ca\u958b\u767c\u6297\u8178\u75c5\u6bd2\u85e5\u7269\u3002\u53e6\u5916\uff0c\u65b0\u51a0\u75c5\u6bd2\u7684\u5168\u7403\u75ab\u60c5\u96d6\u5df2\u544a\u4e00\u6bb5\u843d\uff0c\u4f46\u7531\u65bc\u75c5\u6bd2\u6301\u7e8c\u7a81\u8b8a\uff0c\u75ab\u82d7\u7684\u529f\u6548\u56e0\u6b64\u6703\u6253\u6298\uff0c\u73fe\u6709\u7684\u6297\u75c5\u6bd2\u85e5\u6709\u53ef\u80fd\u7522\u751f\u6297\u85e5\u6027\uff0c\u6709\u8cf4\u6301\u7e8c\u7814\u767c\u6297\u75c5\u6bd2\u85e5\u7269\u3002\u56e0\u6b64\uff0c\u6211\u5011\u4e5f\u7531\u5f9e\u4e8b\u6297\u8178\u75c5\u6bd2\u85e5\u7269\u7684\u6210\u679c\u9032\u4e00\u6b65\u958b\u767c\u5ee3\u6548\u578b\u7684\u51a0\u72c0\u75c5\u6bd2\u85e5\u7269\u3002\u7e3d\u4e4b\u76ee\u524d\u5be6\u9a57\u5ba4\u6709\u4ee5\u4e0b\u4e09\u500b\u7814\u7a76\u65b9\u5411:<\/p><p><em>(A) <\/em><em>\u958b\u767c\u6297<u>\u8178\u75c5\u6bd2<\/u>\u85e5\u7269<\/em><\/p><p>\u00a0 \u00a0 \u672c\u5be6\u9a57\u5ba4\u5df2\u7d93<em>\u61c9\u7528\u87a2\u5149\u5171\u632f\u80fd\u91cf\u8f49\u79fb(fluorescence resonance energy transfer; FRET<\/em><em>)<\/em><em>\u7684\u539f\u7406<\/em>\u767c\u5c55<em>\u6297\u8178\u75c5\u6bd2\u85e5\u7269\u7be9\u9078\u5e73\u53f0<\/em>\uff0c\u5f9e\u5169\u500b\u85e5\u5eab(drug library)\uff0c\u5404\u67091280\u500b\u53ca800\u500b\u5c0f\u5206\u5b50\uff0c\u7be9\u9078\u51fa\u6578\u500b\u5177\u6291\u5236\u8178\u75c5\u6bd2\u8907\u88fd\u7684\u5316\u5408\u7269\uff0c\u90e8\u5206\u85e5\u7269\u662f\u7f8e\u570b\u98df\u54c1\u85e5\u7269\u7ba1\u7406\u5c40\u6279\u51c6\uff0c\u81e8\u5e8a\u4e0a\u7528\u65bc\u6cbb\u7642\u5176\u4ed6\u75be\u75c5\u7684\u85e5\u7269\uff0c\u76ee\u524d\u5176\u4e2d\u4e00\u500b\u85e5\u7269idarubicin\u5df2\u7d93\u95e1\u660e\u5176\u4f5c\u7528\u6a5f\u5236\u662f\u9078\u64c7\u6027\u7d50\u5408\u5230\u8abf\u63a7\u75c5\u6bd2\u8f49\u8b6f\u7684\u75c5\u6bd2\u6838\u91a3\u9ad4\u5167\u5165\u4f4d(internal ribosomal entry site)\uff0c\u4ee5\u6291\u5236\u8178\u75c5\u6bd2\u7684\u8f49\u8b6f\u3002\u53e6\u5916\uff0c\u4e5f\u767c\u73fe\u6578\u500b\u6297\u6182\u9b31\u7684\u81e8\u5e8a\u85e5\u7269\u80fd\u6709\u6548\u6291\u5236\u8178\u75c5\u6bd2\u8907\u88fd\u3002\u7531\u65bc\u9019\u4e9b\u81e8\u5e8a\u7528\u85e5\u7684\u5728\u4eba\u9ad4\u7684\u85e5\u7269\u52d5\u529b\u5b78\u53ca\u5b89\u5168\u6027\u5df2\u7d93\u4e86\u89e3\u76f8\u7576\u6e05\u695a\uff0c\u6709\u5229\u65bc\u820a\u85e5\u65b0\u7528\uff0c\u52a0\u901f\u6297\u8178\u75c5\u6bd2\u85e5\u7269\u7684\u767c\u5c55\u3002\u5f9e\u9019\u5e7e\u500b\u65b0\u627e\u5230\u7684\u85e5\u7269\uff0c\u767c\u73fe\u5176\u4e2d\u6709\u5e7e\u7d44\u4e09\u500b\u81e8\u5e8a\u85e5\u7269\u7684\u7d44\u5408\uff0c\u53ef\u4ee5\u5728\u964d\u4f4e\u85e5\u7269\u6fc3\u5ea6\u52301\/10\u7684\u72c0\u6cc1\u4e0b\uff0c\u6709\u6548\u6291\u5236\u8178\u75c5\u6bd2\u8907\u88fd\u3002\u8a72\u7814\u7a76\u6210\u679c\u5df2\u7d93\u7372\u5f97\u672c\u570b\u3001\u7f8e\u570b\u53ca\u6b50\u76df\u5c08\u5229\u3002<\/p><p><em>(B) <\/em><em>\u958b\u767c\u6297<u>\u51a0\u72c0\u75c5\u6bd2<\/u>\u85e5\u7269<\/em><\/p><p><em>\u00a0\u00a0\u00a0 <\/em><em>\u7531\u65bc<u>\u65b0\u51a0\u75c5\u6bd2<\/u>\u7684\u9ad8\u611f\u67d3\u7387\u53ca\u9ad8\u7f79\u75c5\u81f4\u6b7b\u6027<\/em><em>\uff0c<\/em><em>\u5fc5\u9808\u5728\u7b2c\u4e09\u751f\u5b89\u7b49\u7d1a(<\/em><em>Biosafety level 3, BSL-3<\/em><em>)<\/em><em>\u7684\u5be6\u9a57\u5ba4\u64cd\u4f5c<\/em><em>\uff0c<\/em><em>\u9650\u5236\u4e86\u5176\u7814\u7a76<\/em><em>\u3002<\/em><em>\u6211\u5011\u4ee5\u5169\u500b\u7b56\u7565\u4f86\u7a81\u7834\u7814\u7a76\u969c\u7919:\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/em><\/p><p><em>1. \u5229\u7528\u540c\u5c6c\u51a0\u72c0\u75c5\u6bd2\u79d1<\/em>\uff0c<em>\u5e8f\u5217\u53ca\u8907\u88fd\u6a5f\u5236\u6975\u76f8\u4f3c\u7684229E\u51a0\u72c0\u75c5\u6bd2\u682a\u9032\u884c\u7814\u7a76<\/em><em>\u3002<\/em><em>229E<\/em><em>\u682a\u611f\u67d3\u4eba\u985e\u50c5\u5f15\u8d77\u666e\u901a\u611f\u5192<\/em>\uff0c<em>\u6703\u81ea\u884c\u75ca\u7652<\/em>\uff0c<em>\u56e0\u6b64\u53ef\u5728\u4e00\u822c\u7b49\u7d1a\u7684\u7b2c\u4e8c\u751f\u5b89\u7b49\u7d1a(<\/em><em>BSL-2)<\/em><em>\u5be6\u9a57\u5ba4\u64cd\u4f5c<\/em><em>\u3002\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 <\/em><\/p><p><em>2. <\/em><em>\u4f7f\u7528\u507d\u75c5\u6bd2(Pseudovirus)\u7cfb\u7d71<\/em><em>\u3002<\/em><em>\u5176\u5916\u5c64\u5305\u88f9\u6709\u65b0\u51a0\u75c5\u6bd2\u68d8<\/em><em>(Spike)<\/em><em>\u86cb\u767d\uff0c\u53ef\u6a21\u64ec\u65b0\u51a0\u75c5\u6bd2\u9032\u5165\u7d30\u80de\u7684\u904e\u7a0b\uff0c\u4f46\u611f\u67d3\u7d30\u80de\u5f8c\u7121\u6cd5\u5b8c\u6210\u8907\u88fd\uff0c\u56e0\u6b64\u4e5f\u53ef\u5728\u7b2c\u4e8c\u751f\u5b89\u7b49\u7d1a\u5be6\u9a57\u5ba4\u64cd\u4f5c\uff0c\u53ef\u7528\u4f86\u7be9\u9078\u6291\u5236\u65b0\u51a0\u75c5\u6bd2\u9032\u5165\u7d30\u80de\u7684\u85e5\u7269\u3002 <\/em><em>\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/em><em>\u6211\u5011\u5229\u7528\u6311\u9078\u6297\u8178\u75c5\u6bd2\u7d44\u5408\u85e5\u7269\u5c08\u5229\u7684\u7b56\u7565<\/em>\uff0c<em>\u76ee\u524d\u5df2\u7d93\u767c\u73fe<\/em>\u6578\u7d44\u81e8\u5e8a\u85e5\u7269\u7684\u7d44\u5408\uff0c<em>\u53ef\u5728\u6975\u4f4e\u6fc3\u5ea6\u4e0b<\/em>\u6709\u6548\u6291\u5236229E<em>\u51a0\u72c0\u75c5\u6bd2\u682a<\/em>\u8907\u88fd\u3002\u6211\u5011\u5c07\u8207\u570b\u5167\u7684<em>BSL-3<\/em><em>\u5be6\u9a57\u5ba4\u6d3d\u8ac7\u5408\u4f5c<\/em><em>\uff0c<\/em>\u8a55\u4f30\u78ba\u8a8d\u9019\u5e7e\u500b\u6709\u6548\u7684\u85e5\u7269\u7d44\u5408<em>\u6291\u5236<u>\u65b0\u51a0\u75c5\u6bd2<\/u>\u7684\u6548\u7387<\/em><em>\uff0c<\/em><em>\u4e26\u4e14\u5df2\u7d93\u8457\u624b\u7533\u8acb\u8a72\u7814\u7a76\u6210\u679c\u7684\u5c08\u5229<\/em><em>\u3002<\/em><\/p><p><em>(C)<\/em> <u>\u8178<em>\u75c5\u6bd2\u53ca\u51a0\u72c0\u75c5\u6bd2\u6d3b\u5316\u7d30\u80de\u5167\u9223\u96e2\u5b50\u8a0a\u606f\u50b3\u5c0e\u8def\u5f91\u53ca\u5176\u8abf\u63a7\u75c5\u6bd2\u8907\u88fd\u7684\u6a5f\u5236<\/em><\/u><\/p><p>\u00a0 \u5df2\u77e5\u5305\u62ec\u8178\u75c5\u6bd2<em>\u53ca\u51a0\u72c0\u75c5\u6bd2\u7b49<\/em>\u4e00\u4e9b\u75c5\u6bd2\uff0c\u5728\u611f\u67d3\u5bc4\u4e3b\u7d30\u80de\u5f8c\uff0c\u6703\u9020\u6210\u7d30\u80de\u8cea\u5167\u9223\u96e2\u5b50\u6fc3\u5ea6\u4e0a\u5347\uff0c\u4e14\u9019\u500b\u73fe\u8c61\u662f\u75c5\u6bd2\u8907\u88fd\u6240\u9700\u8981\u7684\u3002\u9032\u4e00\u6b65\u7814\u7a76\u6307\u51fa\u7d30\u80de\u8cea\u5167\u9223\u96e2\u5b50\u4f86\u81ea\u65bc\u7d30\u80de\u5167\u4e3b\u8981\u8caf\u5b58\u9223\u96e2\u5b50\u7684\u5167\u8cea\u7db2\u53ca\u4f86\u81ea\u65bc\u80de\u5916\u74b0\u5883\uff0c\u4f46\u5176\u5206\u5b50\u6a5f\u5236\u4e0d\u660e\u3002\u7531\u65bc\u9223\u96e2\u5b50\u5728\u7d30\u80de\u5167\u5916\u79fb\u52d5\uff0c\u6216\u662f\u5728\u7d30\u80de\u5167\u7684\u80de\u5668\u9593\u53ca\u7d30\u80de\u8cea\u79fb\u52d5\uff0c\u662f\u7531\u8a31\u591a\u9223\u96e2\u5b50\u901a\u9053\u53c3\u8207\uff0c\u800c\u4e14\u9223\u96e2\u5b50\u53ef\u8abf\u63a7\u8a31\u591a\u8a0a\u606f\u50b3\u5c0e\u8def\u5f91\uff0c\u4ee5\u53c3\u8207\u7d30\u80de\u589e\u6b96\uff0c\u7d30\u80de\u9aa8\u67b6(cytoskeleton)\u6539\u8b8a\uff0c\u7d30\u80de\u81ea\u9ad4\u541e\u566c(autophagy)\u3001\u7d30\u80de\u51cb\u4ea1\u53ca\u5f62\u6210\u767c\u708e\u9ad4(inflammasome)\u4ee5\u8abf\u63a7\u514d\u75ab\u53cd\u61c9\u7b49\u7d30\u80de\u529f\u80fd\uff0c\u8a31\u591a\u75c5\u6bd2\u4e5f\u85c9\u8457\u8abf\u63a7\u611f\u67d3\u7d30\u80de\u5167\u7684\u9223\u96e2\u5b50\u6fc3\u5ea6\u4ee5\u5229\u75c5\u6bd2\u672c\u8eab\u7684\u8907\u88fd\u53ca\u6563\u4f48\u3002\u56e0\u6b64\u672c\u5be6\u9a57\u5ba4\u8457\u624b\u7814\u7a76\u8178\u75c5\u6bd2<em>\u53ca\u51a0\u72c0\u75c5\u6bd2<\/em>\u611f\u67d3\u7684\u7d30\u80de\u5167\u9223\u96e2\u5b50\u7684\u79fb\u52d5\uff0c\u662f(1)\u7d93\u7531\u54ea\u4e9b\u9223\u96e2\u5b50\u901a\u9053\uff0c(2)\u6d3b\u5316\u54ea\u4e9b\u7d30\u80de\u8a0a\u606f\u50b3\u5c0e\u8def\u5f91\uff0c\u800c\u6709\u5229\u65bc\u75c5\u6bd2\u672c\u8eab\u7684\u8907\u88fd\u53ca\u6563\u4f48\uff0c\u9020\u6210\u75c5\u6bd2\u7684\u81f4\u75c5\u6027(pathogenesis)\u3002\u95e1\u660e\u5176\u4f5c\u7528\u6a5f\u5236\uff0c\u4e0d\u4f46\u80fd\u66f4\u52a0\u77ad\u89e3\u8178\u75c5\u6bd2<em>\u53ca\u51a0\u72c0\u75c5\u6bd2<\/em>\u8907\u88fd\u53ca\u81f4\u75c5\u7684\u5206\u5b50\u6a5f\u8f49\uff0c\u4e5f\u56e0\u70ba\u8a31\u591a\u9223\u96e2\u5b50\u901a\u9053\u53ca\u8a0a\u606f\u50b3\u5c0e\u8def\u5f91\u5df2\u7d93\u6709\u81e8\u5e8a\u7528\u85e5\uff0c\u9019\u4e5f\u6709\u52a9\u65bc\u958b\u767c\u85c9\u7531\u6291\u5236\u9223\u96e2\u5b50\u901a\u9053\u6216\u9223\u96e2\u5b50\u7d30\u80de\u8a0a\u606f\u50b3\u5c0e\u8def\u5f91\u7684\u5ee3\u6548\u578b\u6297\u75c5\u6bd2\u85e5\u7269\u3002\u76ee\u524d\u5df2\u767c\u8868\u8178\u75c5\u6bd2\u611f\u67d3\u5f8c\u7522\u751f\u9223\u96e2\u5b50\u6d41\u52d5\u5f71\u97ff\u8178\u75c5\u6bd2\u8907\u88fd\u4e4b\u8ad6\u6587\u5982\u4e0b\u3002<\/p><p>\u00a0<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-96b9308 elementor-widget elementor-widget-image\" data-id=\"96b9308\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"1024\" height=\"474\" src=\"https:\/\/dmt.nycu.edu.tw\/wp-content\/uploads\/2025\/11\/\u87a2\u5e55\u64f7\u53d6\u756b\u9762-2025-11-13-113753-1024x474.png\" class=\"attachment-large size-large wp-image-7505\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/dmt.nycu.edu.tw\/wp-content\/uploads\/2025\/11\/\u87a2\u5e55\u64f7\u53d6\u756b\u9762-2025-11-13-113753-1024x474.png 1024w, https:\/\/dmt.nycu.edu.tw\/wp-content\/uploads\/2025\/11\/\u87a2\u5e55\u64f7\u53d6\u756b\u9762-2025-11-13-113753-300x139.png 300w, https:\/\/dmt.nycu.edu.tw\/wp-content\/uploads\/2025\/11\/\u87a2\u5e55\u64f7\u53d6\u756b\u9762-2025-11-13-113753-768x355.png 768w, https:\/\/dmt.nycu.edu.tw\/wp-content\/uploads\/2025\/11\/\u87a2\u5e55\u64f7\u53d6\u756b\u9762-2025-11-13-113753-18x8.png 18w, https:\/\/dmt.nycu.edu.tw\/wp-content\/uploads\/2025\/11\/\u87a2\u5e55\u64f7\u53d6\u756b\u9762-2025-11-13-113753.png 1206w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-5f0d1d5 elementor-widget elementor-widget-text-editor\" data-id=\"5f0d1d5\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>\u5716\u4e00\u3001\u8178\u75c5\u6bd271\u8a98\u5c0e\u7d30\u80de\u8cea\u5167\u9223\u96e2\u5b50(Ca\u00b2\u207a)\u5347\u9ad8\uff0c\u900f\u904e\u6d3b\u5316\u5132\u5b58\u64cd\u4f5c\u6027\u9223\u6d41(SOCE)\u8207\u7c92\u7dda\u9ad4\u9223\u96e2\u5b50\u6d41\u5165\u5f71\u97ff\u75c5\u6bd2\u751f\u547d\u9031\u671f\u7684\u6a5f\u5236\u6a21\u578b\u3002<br \/>\u8178\u75c5\u6bd271\u76842B\u86cb\u767d\u9020\u6210\u5167\u8cea\u7db2\uff08ER\uff09\u4e2d\u9223\u96e2\u5b50\u7684\u6d88\u8017\uff0c\u9032\u800c\u555f\u52d5\u4e86SOCE\u3002\u5728\u611f\u67d3\u5f8c6\u5c0f\u6642 (6 h p.i.)\uff0c\u7d30\u80de\u8cea\u5167\u9223\u96e2\u5b50\u6fc3\u5ea6\u4e0a\u5347\uff0c\u5c0e\u81f4\u7c92\u7dda\u9ad4\u9223\u96e2\u5b50\u4e2d\u5ea6\u589e\u52a0\uff0c\u96a8\u4e4b\u4fc3\u9032\u7c92\u7dda\u9ad4\u96fb\u5b50\u50b3\u905e\u93c8\u8907\u5408\u9ad4\uff08I\u81f3IV\uff09\u7684\u6d3b\u6027\uff0c\u4e26\u63d0\u9ad8ATP\u7684\u751f\u6210\u4ee5\u4f9b\u75c5\u6bd2\u8907\u88fd\u4f7f\u7528\u3002<br \/>\u5728\u611f\u67d3\u5f8c12\u5c0f\u6642\uff0812 h p.i.\uff09\uff0c\u7c92\u7dda\u9ad4\u9223\u96e2\u5b50\u904e\u91cf\u7d2f\u7a4d\u5f15\u767c\u5167\u5728\u6027\u51cb\u4ea1\u9014\u5f91\uff0c\u4fc3\u9032\u75c5\u6bd2\u7d93\u7531\u7d30\u80de\u51cb\u4ea1\u91cb\u653e\u3002AnCoA4\u8207RR\u53ef\u964d\u4f4e\u7d30\u80de\u8cea\u53ca\u7c92\u7dda\u9ad4\u9223\u96e2\u5b50\u7684\u6fc3\u5ea6\uff0c\u5206\u5225\u6e1b\u5c11\u75c5\u6bd2\u7684\u8907\u88fd\u8207\u91cb\u653e\u3002Oligomycin\u5247\u4f5c\u7528\u65bc\u7c92\u7dda\u9ad4\u8907\u5408\u9ad4V\uff08ATP\u5408\u9176\uff09\uff0c\u5f9e\u800c\u6291\u5236\u75c5\u6bd2\u8907\u88fd\u3002RR = ruthenium red. MCU = mitochondrial Ca2+ uniporter.<br \/>mBio (2025): e0371724.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-5f564dd5 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"5f564dd5\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-5995e64\" data-id=\"5995e64\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-7ee39161 elementor-widget elementor-widget-heading\" data-id=\"7ee39161\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">\u5c08\u5229<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2f19a645 elementor-widget elementor-widget-text-editor\" data-id=\"2f19a645\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>\u8178\u75c5\u6bd2<span lang=\"EN-US\">71<\/span>\u578b\u7d44\u5408\u85e5\u7269\u6cbb\u7642<span lang=\"EN-US\">:<\/span><\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-9212abd elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"9212abd\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-66 elementor-inner-column elementor-element elementor-element-b0ecf6f\" data-id=\"b0ecf6f\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-aaf3161 elementor-widget elementor-widget-image\" data-id=\"aaf3161\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"1024\" height=\"741\" src=\"https:\/\/dmt.nycu.edu.tw\/wp-content\/uploads\/2021\/09\/\u622a\u5716-2021-09-07-\u4e0b\u53482.23.21-1024x741.png\" class=\"attachment-large size-large wp-image-2518\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/dmt.nycu.edu.tw\/wp-content\/uploads\/2021\/09\/\u622a\u5716-2021-09-07-\u4e0b\u53482.23.21-1024x741.png 1024w, https:\/\/dmt.nycu.edu.tw\/wp-content\/uploads\/2021\/09\/\u622a\u5716-2021-09-07-\u4e0b\u53482.23.21-300x217.png 300w, https:\/\/dmt.nycu.edu.tw\/wp-content\/uploads\/2021\/09\/\u622a\u5716-2021-09-07-\u4e0b\u53482.23.21-768x556.png 768w, https:\/\/dmt.nycu.edu.tw\/wp-content\/uploads\/2021\/09\/\u622a\u5716-2021-09-07-\u4e0b\u53482.23.21.png 1428w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-inner-column elementor-element elementor-element-1001086\" data-id=\"1001086\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-b95b684 elementor-widget elementor-widget-image\" data-id=\"b95b684\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"699\" height=\"1024\" src=\"https:\/\/dmt.nycu.edu.tw\/wp-content\/uploads\/2021\/09\/\u622a\u5716-2021-09-07-\u4e0b\u53482.23.56-699x1024.png\" class=\"attachment-large size-large wp-image-2519\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/dmt.nycu.edu.tw\/wp-content\/uploads\/2021\/09\/\u622a\u5716-2021-09-07-\u4e0b\u53482.23.56-699x1024.png 699w, https:\/\/dmt.nycu.edu.tw\/wp-content\/uploads\/2021\/09\/\u622a\u5716-2021-09-07-\u4e0b\u53482.23.56-205x300.png 205w, https:\/\/dmt.nycu.edu.tw\/wp-content\/uploads\/2021\/09\/\u622a\u5716-2021-09-07-\u4e0b\u53482.23.56-768x1125.png 768w, https:\/\/dmt.nycu.edu.tw\/wp-content\/uploads\/2021\/09\/\u622a\u5716-2021-09-07-\u4e0b\u53482.23.56.png 834w\" sizes=\"(max-width: 699px) 100vw, 699px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-f69b022 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"f69b022\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-efdc948\" data-id=\"efdc948\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-dfb10b8 elementor-widget elementor-widget-heading\" data-id=\"dfb10b8\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">\u4ee3\u8868\u8457\u4f5c<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-5d8e8cd elementor-widget elementor-widget-text-editor\" data-id=\"5d8e8cd\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Hsu BY, Tsai YH, Weng TC, Szu-Hao Kung*. 2025. Activation of store-operated calcium entry and mitochondrial respiration by enterovirus 71 is essential for efficient virus replication. mBio 16:e0371724.<\/p><p>Weng TC, Hsu BY, Szu-Hao Kung* 2025. Cepharanthine inhibits enterovirus entry by endolysosomal deacidification and exhibits protective activity in vivo. Antimicrob Agents Chemother doi:10.1128\/aac.00764-25:e0076425.<\/p><p>Tseng KC, Hsu BY, Ling P, Lu WW, Lin CW and Szu-Hao Kung* (2022). Antidepressant sertraline is a broad-spectrum inhibitor of enteroviruses targeting viral entry through neutralization of endolysosomal acidification. Viruses. 8; 14 (1):109.<\/p><p>Chen KR, Yu CK, <u>Kung SH<\/u>, Chen SH, Chang CF, Ho TC, Lee YP, Chang HC, Huang LY, Lo\u00a0\u00a0\u00a0\u00a0 SY, Chang JC, Ling P. (2018). Toll-like receptor 3 is involved in detection of\u00a0\u00a0\u00a0 enterovirus A71 infection and targeted by viral 2A protease. <strong>Viruses<\/strong><strong>.<\/strong> 2018.10(12). E689.<\/p><p>Lu WW, Kung FY, Deng PA, Lin YC, Lin CW, <u>Kung SH.<\/u> (2017). Development of a fluorescence resonance energy transfer\u2013based intracellular assay to identify novel enterovirus 71 antivirals. <em>Arch. Virol.<\/em> 162 (3): 713-720.<\/p><p>Lu CY, Chang YC, Hua CH, Chuang C, Huang SH, Kung SH, Hour MJ, Lin CW. (2017). Tubacin, an HDAC6 Selective Inhibitor, Reduces the Replication of the Japanese Encephalitis Virus via the Decrease of Viral RNA Synthesis. International Journal of Molecular Science 18(5):E954.<\/p><p>Hou HY, Lu WW, Wu KY, Lin CW, <u>Kung SH<\/u> (2016). Idarubicin is a broad-spectrum enterovirus replication inhibitor that selectively targets the virus internal ribosomal entry site. <em>J Gen Virol<\/em><em>.<\/em> 97(5): 1122-33.<\/p><p>Syu BY, Chao YT, Ou PT, <u>Kung SH<\/u>*. The CMP<sup>TM<\/sup> inactive virus transport medium can inactivate human coronavirus. <em>Journal of Testing and Quality Assurance<\/em>, 2021: 10:53-59.<\/p><p>Wang CY, Huang AC, Hour MJ, Huang SH, <u>Kung SH<\/u>, C Chen CH, Chen IC, Chang YS, CW Lin (2015). Antiviral Potential of a Novel Compound CW-33 against Enterovirus A71 via Inhibition of Viral 2A Protease. Viruses. 7, 3155-3171.<\/p><p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/27164085\/\">SARS Coronavirus Papain-Like Protease Inhibits the TLR7 Signaling Pathway through Removing Lys63-Linked Polyubiquitination of TRAF3 and TRAF6.<\/a> Li SW, Wang CY, Jou YJ, Huang SH, Hsiao LH, Wan L, Lin YJ,\u00a0Kung SH, Lin CW. Int J Mol Sci. 2016 May 5;17(5):678.<\/p><p>Lu JR, Lu WW, Lai JZ, Tsai FL, Wu SH, Lin CW, <u>Kung SH.<\/u> (2013) <a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/23515028\">Calcium flux and calpain-mediated activation of the apoptosis-inducing factor contribute to enterovirus 71-induced apoptosis.<\/a> <em>J Gen Virol<\/em><em>.<\/em> 94(7):1477-85.<\/p><p>Lu WW, Sun JR, SS Wu, Lin WH, <u>Kung SH <\/u>(2011). A dual reporter cell assay for identifying serotype and drug susceptibility of herpes simplex virus. <em>Analytical Biochemistry<\/em>. Aug 15;415(2):97-104.<\/p><p>Li SW, Lai CC, Ping JF, Tsai FJ, Wan L, Lin YJ, <u>Kung SH<\/u>, Lin CW. (2011). <a href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/21270289\">Severe acute respiratory syndrome coronavirus papain-like protease suppressed alpha interferon-induced responses through downregulation of extracellular signal-regulated kinase 1-mediated signalling pathways.<\/a> <em>Journal of General Virology<\/em>. 92:1127-40.<\/p><p>Hsu YY, Liu YN, Lu WW, <u>Kung SH.<\/u> (2009). Visualizing and quantifying the differential cleavages of the eukaryotic translation initiation factors eIF4GI and eIF4GII in the enterovirus-infected cell. Biotechnol Bioeng. 104(6):1142-52.<\/p><p>Tsai MT, Cheng YH, Liu YN, Liao NC, Lu WW, <u>Kung SH.<\/u> (2009). Real-time monitoring of human enterovirus (HEV)-infected cells and anti-HEV 3C protease potency by fluorescence resonance energy transfer. <em>Antimicrob Agents Chemother.<\/em> 53(2):748-755.<\/p><p>Hsu YY, Liu YN, Wang W, Kao FJ, and <u>Kung SH<em>.<\/em><\/u> (2007).<em> In Vivo<\/em> Dynamics of Enterovirus Protease Revealed by Fluorescence Resonance Emission Transfer (FRET) based on a novel FRET pair. <em>Biochem Biophys Res Commun. <\/em>353, 939\u2013945.<\/p><p><a href=\"http:\/\/www.ncbi.nlm.nih.gov\/sites\/entrez?Db=pubmed&amp;Cmd=ShowDetailView&amp;TermToSearch=17488328&amp;ordinalpos=1&amp;itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum\">Kung SH, Wang SF, Huang CW, Hsu CC, Liu HF, Yang JY.<\/a> (2007). Genetic and antigenic analyses of enterovirus 71 isolates in Taiwan during 1998-2005. <em>Clin Microbiol Infect.<\/em> 13(8):782-7.<\/p><p><a href=\"http:\/\/www.ncbi.nlm.nih.gov\/sites\/entrez?Db=pubmed&amp;Cmd=ShowDetailView&amp;TermToSearch=17477731&amp;ordinalpos=3&amp;itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum\">Ghukasyan V, Hsu YY, Kung SH, Kao FJ.<\/a> (2007). Application of fluorescence resonance energy transfer resolved by fluorescence lifetime imaging microscopy for the detection of enterovirus 71 infection in cells. <em>J Biomed Opt. <\/em>12(2):024016.<\/p><p><a href=\"http:\/\/www.ncbi.nlm.nih.gov\/entrez\/query.fcgi?cmd=Retrieve&amp;db=pubmed&amp;dopt=Abstract&amp;list_uids=15530419\">Lu WW, Hsu YY, Yang JY, Kung SH.<\/a> (2004). Selective inhibition of enterovirus 71 replication by short hairpin RNAs. <em>Biochem Biophys Res Commun.<\/em> 325(2):494-9.<\/p><p>1998-2002<\/p><p>Kuo YC, Lin LC, Tsai WJ, Chou CJ, <u>Kung SH,<\/u> Ho, YH.. Samarangenin B from Limonium sinense suppresses herpes simplex virus type 1 replication in Vero cells by regulation of viral macromolecular synthesis. Antimicrobial Agents and Chemotherapy 2002;46(9):2854-2864<\/p><p>Y-C Wang, C-L Kao, W-T Liu, J-R Sun, Y-E Tai, and <u>Kung SH<\/u>.. A Cell Line That Secretes Inducibly a Reporter Protein for Monitoring Herpes Simplex Virus Infection and Drug Susceptibility. Journal of Medical Virology 2002;68(4):599-605.<\/p><p>Kuo, RL, <u>Kung, S.H<\/u>., Hsu, YY, and Liu, WT. Infection with Enterovirus 71 or expression of its 2A protease induces apoptotic cell death. Journal of General Virology 2002;83:1367-1376.<\/p><p>Tai, H.Y., Sun, K.H., <u>Kung, S.H<\/u>., Liu, W.T. A quantitative assay for measuring human foamy virus using an established indicator cell line. Journal of Virological Methods 2001;94:155-162.<\/p><p>Liu, W.T., Sun, JR, Lin, CH, Kuo RL, and <u>Kung SH<\/u>.. An indicator cell assay for detection of human cytomegalovirus based on enhanced green fluorescent protein. Journal of Virological Methods 2001;96:85-92.<\/p><p><u>Kung, S.H<\/u>., Wang, Y.C., Lin, C.H., Kuo, R.L., and Liu, W.T. Rapid diagnosis and quantification of herpes simplex virus with a green fluorescent protein reporter system. Journal of Virological Methods 2000;90:205-212.<\/p><p><u>Kung, S.H<\/u>., Hagstrome, J.N., Cass, D., Tai, S.J., Lin, H.F., Stafford, D. and High K.A.. Human coagulation factor IX corrects the bleeding diathesis of mice with hemophilia B. <em>Blood<\/em> 1998;91:784-790.<\/p><p>Nakai, H., Herzog, R.H., Hagstrome, J.H., Walter, J.H., <u>Kung, S.H<\/u>., Yang, E.Y., Tai, S.H., Iwaki, Y., Kurtzman, G.J., Fisher,K.J., Colosi,P., Couto, L.B., and High, K.A.. Adeno-associated viral vector-mediated gene transfer of human blood coagulation factor IX into mouse liver. <em>Blood <\/em>1998;91:4600-4607.<\/p><p>Herzog, R.W., Hagstrome, J.N., <u>Kung, S.H<\/u>., Tai, S.J., Wilson, J.M., Fisher, K. J., and High, K.A. Stable gene transfer and expression of human blood coagulation factor IX after intramuscular injection of recombinant adeno-associated virus. <em>Proceedings of the national academy of sciences of the United States of America<\/em> 1997;94:5804-5809.<\/p><p><u>Kung, S.H<\/u>. and Medveczky, P. Identification of a Herpesvirus saimiri cis-acting DNA fragment that permits stable replication of episomes in transformed T cells. <em>Journal of Virology<\/em> 1996;70:1738-1744.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>\u9f94\u601d\u8c6a Szu-Hao\u00a0Kung \u806f\u7d61\u65b9\u5f0f \u260f\u00a002-28267034 \u2709\u00a0szkung@nycu.edu.t &hellip;<\/p>\n<p class=\"read-more\"> <a class=\"\" href=\"https:\/\/dmt.nycu.edu.tw\/en\/%e9%be%94%e6%80%9d%e8%b1%aa\/\"> <span class=\"screen-reader-text\">\u9f94\u601d\u8c6a<\/span> Read More &raquo;<\/a><\/p>","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"site-sidebar-layout":"default","site-content-layout":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":""},"_links":{"self":[{"href":"https:\/\/dmt.nycu.edu.tw\/en\/wp-json\/wp\/v2\/pages\/291"}],"collection":[{"href":"https:\/\/dmt.nycu.edu.tw\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/dmt.nycu.edu.tw\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/dmt.nycu.edu.tw\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/dmt.nycu.edu.tw\/en\/wp-json\/wp\/v2\/comments?post=291"}],"version-history":[{"count":51,"href":"https:\/\/dmt.nycu.edu.tw\/en\/wp-json\/wp\/v2\/pages\/291\/revisions"}],"predecessor-version":[{"id":7511,"href":"https:\/\/dmt.nycu.edu.tw\/en\/wp-json\/wp\/v2\/pages\/291\/revisions\/7511"}],"wp:attachment":[{"href":"https:\/\/dmt.nycu.edu.tw\/en\/wp-json\/wp\/v2\/media?parent=291"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}